By Y. Kitagawa, T. Matsuda, S. Iijima
Animal phone expertise is a growing to be self-discipline of telephone biology which goals not just to appreciate buildings, services and behaviors of differentiated animal cells but in addition to examine their skills for use for business and scientific reasons. The target of animal mobile know-how comprises accomplishments of clonal growth of differentiated cells with beneficial skill, optimization in their tradition stipulations, modulation in their skill for construction of medically and pharmaceutically very important proteins, and the applying of animal cells to gene remedy and synthetic organs. This quantity supplies the readers an entire evaluation of the current state-of-the-art in Japan. The court cases might be important for phone biologists, biochemists, molecular biologists, immunologists, biochemical engineers and different disciplines relating to animal mobile tradition, operating both in educational environments or in industries of biotechnology and pharmacy.
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Additional info for Animal Cell Technology: Basic & Applied Aspects
M. (1994) Generation of cytotoxic T-lymphocytes to a selfpeptide/class I complex: a model for peptide-mediated tumor rejection. Cancer Res. 54, 204-208. , Seliger. H. and Boon, T. (1994) Two tyrosinase nonapeptides recognized on HLA-A2 melanomas by autologous cytolytic T lymphocytes. Eur. J. Immunol. 24, 759-764. This page intentionally left blank.
ENHANCEMENT OF THE ACTIVITY OF ANTISENSE OLIGONUCLEOTIDES Glycosylated polylysines of relatively large size were shown to give stable complexes with oligonucleotides containing about 20 bases. In addition, when the ratio of charges born by the glycosylated polymers (cation) and of those born by the oligonucleotides is close to 1, the efficiency is optimal. This was clearly shown by using a phosphorothioate oligonucleotide, complementary of a sequence in an intron close to the 3' end of the ICAM-1 mRNA.
10, 15–21. © 1999 Kluwer Academic Publishers. Printed in the Netherlands. where 16 thiocarbamy le residues, respectively. The apparent affinity of neoglycoproteins for lectins is much higher (more than 1000) than the corresponding free sugar, for instance 500 nM instead of 2 mM. 2. , 1989). This poly-L-lysine, para- toluene sulfonate is soluble in organic solvent (such as N, N-dimethyformamide) allowing a very efficient substitution by either glycosylphenyl-isothiocyanate or fluoresceinylisothiocyanate or both.