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Additional info for Animal Cell Technology: Basic & Applied Aspects: Proceedings of the 17th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT), ... Cell Technology: Basic & Applied Aspects)
Immunol. 1994; 93 (1) Part 1: 93-99. 15. de Jong, E. , Martens, B. P. , Kapsenberg, M. , Penninks, A. , and Weirenga, E. , Allergen, IgE, mediators, Inflammatory mechanisms: Food allergen (peanut)-specific Th2 clones generated from the peripheral blood of a patient with peanut allergy. J. Allergy Clin. Immunol. 1996; 98 (1): 73-81. 16. Higgins J. , Lamb, J. , Lake, R. , and O’Hehir, R. , Polyclona and clonal + analysis of human CD4 T-lymphocyte response to nut extracts. Immunol. 1995; 84: 91-97.
RESULTS AND DISCUSSION A schematic diagram and the representation of the in vitro coculture system used in this study are shown in Fig. 1. The two different types of cell used with this method were not in contact with each other, enabling the cross talk to be observed between the two types of cell via the soluble factors secreted from each cell line. Semi-permeable membrane Human intestinal Caco-2 cell menolayers Human macrophage like THP-1 cells Figure 1. Caco-2 cells were cultured in 12-well cell culture inserts on a semipermeable support membrane precoated with type-I collagen and formed a monolayer of non-overlapping and contact-inhibited cells.
And Helm, R. , Identification and characterization of a second major peanut allergen, Arah II, with use of the sera of patients with atopic dermatitis and positive peanut challenge. J. Allergy Clin. Immunol. 1992; 90 (6) Part 1: 962-969 12. Rabjohn, P. , Burks, A. W. and Bannon, G. A. Molecular cloning and epitope analysis of athe peanut allergen Ara h 3. J. Clin. Invest. 1999; 103 (4): 535-542. 13. Burks, A. , Stanley, S. , Helm, R. , and Bannon, G. A. Mapping and mutational analysis of the IgE-binding epitopes of Ara h1, a legume vicillin protein and a major allergen in peanut hypersensitivity.