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By Minoru Ueda (Ed.)

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By Minoru Ueda (Ed.)

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Trends Cell Bio. 19: 329,1999 8. Zakian VA. Structure and function of telomeres. Annu Rev Genet. 23: 579,1989 9. Olovnikov AM. Principle of marginotomy in template synthesis of polynucleotides. Dokl Akad Nauk Sssr. 201: 1496,1971 10. Watson JD. Origin of concatameric T7 DNA. Nat New Biol. 239: 197,1972 11. Harley CB, Futcher AB, Greder CW. Telomeres shorten during aging of human fibroblasts. Nature. 345: 458,1990 14 Applied Tissue Engineering 12. Allsopp RC, Vaziri H, Patterson C. Telomere length predicts replicative capacity of human fibroblasts.

Briefly, the blood collected was first centrifuged at 1500 rpm for 10 minutes. Subsequently, the yellow plasma (containing the buffer coat that contained platelets and leukocytes) was removed. The second centrifugation was conducted at 3500 rpm for 5 minutes to combine platelets with a single pellet. The plasma supernatant, which was platelet-poor plasma and contained a relatively small number of cells, was removed. The resulting pellet of platelets, the buffer coat/plasma fraction (PRP), was resuspended in remaining 20 ml of the plasma before use in the platelet gel.

Burns. 15: 303,1989 2. Teepe RGC, Koebrugge EJ, Ponec M. Fresh versus cryopreserved cultured allografts for the treatment of chronic skin ulcers. Br J Dermatol. 122: 81,1990 3. Hata K, Kagami H, Ueda M. An experimental study of cultured epithelial grafting using oral mucosal cells. Fabrication of cultured epithelium and its morphological analysis. J Jpn Stomatol Soc. 43: 416,1994 4. Hata K, Kagami K, Ueda M. Matsuyama, M. The characteristics of cultured mucosal cell sheet as material for grafting; Comparison with cultured epidermal cell sheet.

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