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Extra resources for Current Topics in Biochemistry
The initial steps in the catabolism of gangliosides G and . cells. Since all of the gangliosides whose oligo saccharide chain is longer than lactose, are lacking, it was anticipated that something was wrong with the addition of the molecule of N-acetylgalactosamine. The reaction in question is the transfer of N-acetyl galactosamine from UDP-N-acetylgalaetosamine to hematoside (Fig. 3 0 ) . The activity of this aminosugar D l a CER - G L U - G A L - N A N A + UDP - GALNAc — • CER-GLU-GAL-GALNAc + UDP NANA Fig.
W . ) ; however, it can be dissociated by various techniques to subunits as small f 56 CURRENT TOPICS IN BIOCHEMISTRY as 32-36S (3 χ 1 0 ^ m . w . ) . Nevertheless, even if the true molecular weight of the genome is 3 χ 1 0 ^ , a m o l ecular weight of 3 χ 1 0 ^ for the product is still far too small. It is therefore not clear whether the en tire genome is being copied or whether only portions of the genome are copied. Duesberg (7) did the following experiment, which suggests that the former alternative is the case.
Science, 1 5 5 , 86 (1967). 15. , Sphingolipid 46 CURRENT TOPICS IN BIOCHEMISTRY hydrodases in leukemic leukocytes. 27, 1312 (1967). Cancer R e s . , 16. , Gaynor, E . , Gaucher cells in chronic myelogenous leukemia: an acquired abnormality. Blood, 3 3 , 379 (1969). 17. , Preparation of radioactive TaySachs ganglioside labeled in the sialic acid moiety. J. Lipid R e s . , 1 1 , 144 (1970). 18. , Studies on the metabolism of TaySachs ganglioside. , 2 8 , 596 (1969) . 19. , Demonstration of an alteration of ganglioside metabolism in Tay-Sachs disease.