Download Electrochemical Analysis of Proteins and Cells by Genxi Li, Peng Miao PDF

By Genxi Li, Peng Miao

Electrochemical research of Proteins and Cells offers the amazing development remodeled the years within the electrochemical research of proteins and cells, because of the swift improvement of protein electrochemistry including similar applied sciences comparable to floor amendment, molecular acceptance, molecular meeting, and nanotechnology. As an interdisciplinary box combining electrochemistry, analytical chemistry, biochemistry, biophysics, biomedicine and fabric technological know-how, the electrochemical research of proteins and cells has attracted extensive and broad study curiosity. the most emphasis of this ebook is at the ideas of electrochemical ideas and the sensible application of comparable detection structures, that's of significant value in all organic sciences, resembling phone biology and molecular biology, in addition to in biomedical fields like melanoma learn. This short bargains an updated, easy-to-follow presentation of modern advances at the topic and will function a complement for graduate-level classes in analytical chemistry, biochemistry, biophysics, biotechnology, biomedical engineering, and so forth. it will probably additionally aid younger scientists get an summary of this topic.

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By Genxi Li, Peng Miao

Electrochemical research of Proteins and Cells offers the amazing development remodeled the years within the electrochemical research of proteins and cells, because of the swift improvement of protein electrochemistry including similar applied sciences comparable to floor amendment, molecular acceptance, molecular meeting, and nanotechnology. As an interdisciplinary box combining electrochemistry, analytical chemistry, biochemistry, biophysics, biomedicine and fabric technological know-how, the electrochemical research of proteins and cells has attracted extensive and broad study curiosity. the most emphasis of this ebook is at the ideas of electrochemical ideas and the sensible application of comparable detection structures, that's of significant value in all organic sciences, resembling phone biology and molecular biology, in addition to in biomedical fields like melanoma learn. This short bargains an updated, easy-to-follow presentation of modern advances at the topic and will function a complement for graduate-level classes in analytical chemistry, biochemistry, biophysics, biotechnology, biomedical engineering, and so forth. it will probably additionally aid younger scientists get an summary of this topic.

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Reprinted from Ref. [87], Copyright 2011, with permission from Elsevier Protein kinase–catalyzed phosphorylation plays a significant role in many vital biological processes including cell signal transduction, cell proliferation and differentiation [85, 86]. Tyrosine kinases are a subclass of protein kinase, which can catalyze the reaction of transferring a phosphate group from ATP to a protein. Yang et al. [87] have made use of the catalytic activity of tyrosine kinases to develop a label-free electrochemical method to assay the activity of the enzyme.

The biosensor also allows the estimation of semi-quantitative antibody contents in thirty-minute total assay time without the interference of complex matrices. The p53 protein is a kind of tumor suppressor protein with many functions and binding partners in cells. It acts as a cell guardian in cellular proliferation. Therefore, Yeo et al. [46] have developed a selective platform for electrochemically monitoring the cellular p53 concentration. They firstly immobilize the antibody molecules on an (R)-lipo-diaza-18-crown-6 SAM-modified gold electrode.

46] have developed a selective platform for electrochemically monitoring the cellular p53 concentration. They firstly immobilize the antibody molecules on an (R)-lipo-diaza-18-crown-6 SAM-modified gold electrode. The capture of p53 protein can then modulate the charge transfer, which can be monitored by electrochemical impedance spectroscopy. This selective detection of p53 protein can also be used to indicate cancers and other cellular malfunctions. Carcinoembryonic antigen (CEA) is a glycoprotein involved in cell adhesion, which is a kind of broad-spectrum tumor marker.

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