Download Enzyme Catalysis in Organic Synthesis: A Comprehensive by Karlheinz Drauz, Herbert Waldmann PDF

By Karlheinz Drauz, Herbert Waldmann

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By Karlheinz Drauz, Herbert Waldmann

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Extra info for Enzyme Catalysis in Organic Synthesis: A Comprehensive Handbook 2nd

Example text

If such an effect is noted, the activator should be added in saturating amounts. Special effects are observed in the kinetics of allosteric enzymes. A typical sigmoidal curve describing reaction rate as a function of substrate concentration is presented in Fig. 1-11. Binding of an effector to the regulatory center alters the reaction rate very efficiently and subtly and is often used in nature to divert the metabolic flow into different directions at branching points. Such a response is important for living systems, but rarely will be seen with enzymes employed in organic synthesis.

Lalonde USA Division of Chemistry Bio-organicChemistry Vrije University Amsterdam De Boelelaan 1083 1081 H V Amsterdam The Netherlands Andreas Liese RebekkaJ. Parker Forschungszentrum Jiilich GmbH I BT Leo-Brandt-Strage D-52428 Jiilich Germany School of Chemistry University of Bath Claverton Down Bath BA2 7AY United Kingdom Altus Biologics Inc. O. Box 1 2600 MA Delft The Netherlands Reinhard Reents Max-Planck-Institutfur Molekulare Physiologie Abteilung Chemische Biologie Otto-Hahn-Strage 11 44227 Dortmund Germany Christoph Syldatk Institute for Bioprocess Engineering University of Stuttgart Department of Microbial Physiology Allmandring 31 70569 Stuttgart Germany Peter Rasor Fritz Theil Industrial Biochemicals Business BB-PS, Roche Molecular Biochemicals Roche Diagnostic GmbH Nonnenwald 2 82372 Penzberg Germany ASCA Angewandte Synthesechemie Adlershof GmbH Richard-Willstatter-Strage 12 12489 Berlin Germany I.

Carboxypeptidase A hydrolyzes proteins sequentially starting from the free carboxyl terminus. The enzyme preferentially cleaves hydrophobic amino acids. Already in 1967 the three-dimensional structure had been determined with high resolution by W. Lipscomb and his group. In the meantime much is known about the catalybc mechanism. The essential features are discussed here briefly to improve the understanding of how enzymes work. Two aspects of enzymatic catalysis can be illustrated by this example: 1.

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