By Bernard R. Glick
The molecular biotechnology revolution -- Molecular biotechnology organic platforms -- DNA, RNA, and protein synthesis -- Recombinant DNA know-how -- Chemical synthesis, sequencing, and amplification of DNA -- Manipulation of gene expression in prokaryotes -- Heterologous protein construction in eukaryotic cells -- Directed mutagenesis and protein engineering -- Molecular biotechnology of microbial platforms -- healing brokers -- Vaccines -- Synthesis of business items through recombinant microorganisms -- Bioremediation and biomass usage -- Plant growth-promoting micro organism -- Microbial pesticides -- Large-scale creation of proteins from recombinant microorganisms -- Genetic engineering of crops: technique -- Genetic engineering of crops: purposes -- Transgenic animals -- Human molecular genetics -- Regulating and patenting molecular biotechnology -- Patenting biotechnology innovations
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Additional resources for Molecular Biotechnology: Principles and Applications of Recombinant DNA
2. Detection of metal resistance genes in environmental isolates. For example, a probe was constructed for detecting the mer operon, which controls mercury detoxiﬁcation. 3. Tracking of speciﬁc bacteria in the environment. , nitrogen ﬁxing bacteria or bacteria capable of degrading a speciﬁc substrate) and genetically engineered microbes in water, wastewater, biosolids, and soils. 2 RNA-Based Methods. Ribosomal RNA (rRNA) is a good target to probe because of the large number of ribosomes in living cells.
Yeast) can grow under facultatively anaerobic conditions. Fungi are signiﬁcant components of the soil microﬂora, and a great number of fungal species are pathogenic to plants, causing signiﬁcant damage to agricultural crops. A limited number of species are pathogenic to humans and cause fungal diseases called mycoses. Airborne fungal spores are responsible for allergies in humans. , penicillin). In Chapter 12 we will discuss their role in composting. Identiﬁcation of fungi is mainly based on the type of reproductive structure.
Their cell walls do not contain peptidoglycan. Their rRNA is different from eukaryotic and prokaryotic rRNA. It appears that archaea are more closely related to eukaryotes than to bacteria. As regards their metabolism, archaea may range from organotrophs (use of organic compounds as a source of carbon and energy) to chemoautotrophs (use of CO2 as a carbon source). Most of the archaea live in extreme environments and are called extremophiles. They include the thermophiles, hyperthermophiles, psychrophiles, acidophiles, alkaliphiles, and halophiles.