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By Anatoly B. Kolomeisky

Explores the mechanisms of mobile functioning linked to numerous particular enzymatic molecules referred to as motor proteins. -- jacket.

summary: Explores the mechanisms of mobile functioning linked to numerous particular enzymatic molecules known as motor proteins. -- jacket

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By Anatoly B. Kolomeisky

Explores the mechanisms of mobile functioning linked to numerous particular enzymatic molecules referred to as motor proteins. -- jacket.

summary: Explores the mechanisms of mobile functioning linked to numerous particular enzymatic molecules known as motor proteins. -- jacket

Show description

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There are several single-molecule methods that have been successfully applied for analyzing properties of molecular motors. Optical-trap spectroscopy, which is one of the most powerful and widely utilized methods, is based on the ability to chemically connect a single motor protein molecule to a bead that might be controlled by a calibrated external non-uniform laser field (Howard 2001, Veigel and Schmidt 2011, Greenleaf et al. 2007)[68, 175, 63]. A schematic view of experiments that use the optical-trapping systems is presented in Fig.

2007) [95, 4]. This motion is supported by flagella, which can be viewed as long helical filaments that extend from the cell main body, and it is driven by BFM. A typical bacterium with multiple flagella is shown in Fig. 6. Note that these cellular extensions might be larger in length than the cell itself! The energy source for these motors comes from the difference in ion concentrations (H+ or Na+ ) inside and outside of the cellular membrane, so these species can also be considered as electrochemical motors.

1 HISTORY OF MOTOR PROTEINS Since discovery of the microscope in the 17th century by Dutch tradesmanturned-scientist Antony van Leeuwenhoek (see Fig. 1), people started to look in great detail into phenomena that are taking place in tiny objects such as drops of water, leaves, insects, plants, soil, etc. It was realized then that processes in the microscopic world seem to be very complex and chaotic, and that biological systems are surprisingly dynamic. It was the beginning of a long quest for understanding mechanisms that generate forces and motion in living organisms.

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