By Satyajit D. Sarker
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Additional resources for Natural Products Isolation (Methods in Biotechnology)
Solvent-extraction methods available for the initial and bulk laboratoryscale extraction of natural products from plant and microbial sources (solid–liquid extraction mainly) are presented in this chapter. Focus is also made on particular procedures, useful for removing unwanted interfering contaminants and enriching the extract with desired metabolites. Various other available natural product extraction methods are discussed in Chapters 3, 10, and 13–16. Initial and Bulk Extraction 29 2. 1. Extraction of Plant Natural Products Plants are complex matrices, producing a range of secondary metabolites with different functional groups and polarities.
2000) Recent Research Developments in Phytochemistry. vol. 4 (Pandalai, S. ) Research Signpost, India, pp. 143–152. Natural Product Isolation 25 19. Sarker S. , et al. (2003) Screening the extracts of the seeds of Achillea millefolium, Angelica sylvestris and Phleum pratense for antibacterial, antioxidant activities and general toxicity. Orient. Phar. Exp. Med. 33, 157–162. 20. Hostettmann, K. -L. ), Taylor and Francis, New York, USA, pp. 31–68. 21. Viletinck, A. J. and Apers, S. ), Taylor and Francis, New York, USA, pp.
The initial choice of the most appropriate solvent is based on its selectivity for the substances to be extracted. , alkanes, fatty acids, pigments, waxes, sterols, some terpenoids, alkaloids, and coumarins). , ﬂavonoid glycosides, tannins, some alkaloids). , glycosides, quaternary alkaloids, tannins) (see Chap. 16). A selective extraction can also be performed sequentially with solvents of increasing polarity. This has the advantage of allowing a preliminary separation of the metabolites present in the material within distinct extracts and simpliﬁes further isolation (7).