Download Nucleic Acids in Immunology: Proceedings of a Symposium Held by Michael Heidelberger (auth.), Otto J. Plescia, Werner Braun PDF

By Michael Heidelberger (auth.), Otto J. Plescia, Werner Braun (eds.)

Two fields have performed a number one position in biomedical examine in recent times, the biochemistry of nucleic acids and immunology. but, apart from these features that have been excited by antibody synthesis for example of protein synthesis, there has been until eventually lately an absence of direct organization among the 2 fields. until eventually relatively lately the antigenicity of nucleic acids used to be nonetheless unsure and certainly represented a debatable topic. additionally, the precise position of a few of the nucleic acids in quite a few levels of antibody synthesis used to be doubtful. those skepticisms and uncertainties disappeared speedily within the previous few years. New experi­ psychological techniques introduced the belief that nucleic acids, below acceptable stipulations, are certainly immunogenic, and that the ensuing antibodies can provide new instruments for the exploration of the mplecular constitution of the all-important relatives of nucleic acid molecules. ' whilst, the popularity of the antigenicity of nucleic acids introduced a brand new point of knowing to convinced auto-immune ailments and seasoned­ vided new fabric for the exploration of the function of a service in immune responses. aspect by way of part with this improvement was once the virtually explosive improvement of recent experimental ways and new principles bearing on the matter of antibody formation. Nucleic acids of their numerous kinds have been well-known as taking part in an anticipated significant position within the activation of antibody-forming cells. maybe much less to be anticipated used to be the position they could playas non-specific stimulators of antibody formation.

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By Michael Heidelberger (auth.), Otto J. Plescia, Werner Braun (eds.)

Two fields have performed a number one position in biomedical examine in recent times, the biochemistry of nucleic acids and immunology. but, apart from these features that have been excited by antibody synthesis for example of protein synthesis, there has been until eventually lately an absence of direct organization among the 2 fields. until eventually relatively lately the antigenicity of nucleic acids used to be nonetheless unsure and certainly represented a debatable topic. additionally, the precise position of a few of the nucleic acids in quite a few levels of antibody synthesis used to be doubtful. those skepticisms and uncertainties disappeared speedily within the previous few years. New experi­ psychological techniques introduced the belief that nucleic acids, below acceptable stipulations, are certainly immunogenic, and that the ensuing antibodies can provide new instruments for the exploration of the mplecular constitution of the all-important relatives of nucleic acid molecules. ' whilst, the popularity of the antigenicity of nucleic acids introduced a brand new point of knowing to convinced auto-immune ailments and seasoned­ vided new fabric for the exploration of the function of a service in immune responses. aspect by way of part with this improvement was once the virtually explosive improvement of recent experimental ways and new principles bearing on the matter of antibody formation. Nucleic acids of their numerous kinds have been well-known as taking part in an anticipated significant position within the activation of antibody-forming cells. maybe much less to be anticipated used to be the position they could playas non-specific stimulators of antibody formation.

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Read Online or Download Nucleic Acids in Immunology: Proceedings of a Symposium Held at the Institute of Microbiology of Rutgers, The State University PDF

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Additional resources for Nucleic Acids in Immunology: Proceedings of a Symposium Held at the Institute of Microbiology of Rutgers, The State University

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Taken from Halloran and Parker (1966b). 26 C. W. Parker and M. J. Halloran curred in vivo or in vitro, during conjugation or incorporation of the conjugate into adjuvant. 2. The Basis for Immunization While the stable association of DNA with protein would be expected to account for the formation of anti-DNA antibody, at least one alternative explanation must be considered. The water soluble carbodiimides, which are currently available commercially and have been used in our conjugation studies, contain tertiary amino groups.

4) The relative stability of nucleotide-protein bonds to chemical hydrolysis. On the basis of the information obtained, it seemed clear that phosphodiester and carboxylate ester bonds were not involved to any significant extent in conjugation. Considering the evidence as a whole, and in particular the finding that extensive conjugation of mononucleotides occurred with primary amino groups of polylysine, the predominant bond appeared to be of the phosphoramidate type (pathway 2). However, rigorous chemical proof for this linkage was not obtained.

Shortly thereafter, Levine et al. (1960) obtained convincing evidence that antibodies with nucleic acid specificity were formed in response to immunization with T-even coliphage extracts. ) 1962). } 1962). • Supported by grants of the U. S. Public Health Service. Career Development Awardee of the National Institute of Allergy and Infectious Disease. t 18 Nucleic Acid-Specific Antibodies 19 I. THE PREPARATION OF DNA· AND NUCLEOTIDE· PROTEIN CONJUGATES A. RATIONALE With available evidence that there was no fundamental barrier to the formation of antibodies to nucleic acids, the question arose as to whether or not such antibodies might be useful for the elucidation of RNA and DNA structure.

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