By Jacek J. Hawiger (Eds.)
This quantity features a entire number of tools for the isolation of platelet membranes, subcellular organelles, the cytoskeleton, and for the assay and purification of platelet receptors.
* Platelets are mobile components with the top density of receptors according to membrane floor sector fascinated with binding of adhesive molecules, clotting, enzymes, and vasoactive amines
* Platelets are crucial for the arrest of bleeding and for formation of intravascular thrombi contributing to center assaults, strokes, and disseminated intravascular coagulation, and are desirous about immune advanced disease
* Platelets are priceless for learning the methods of mobile adhesion, neurotransmitter uptake, stimulus-response coupling, together with sign transduction and secretion
Read or Download Platelets: Receptors, Adhesion, Secretion Part B PDF
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Additional resources for Platelets: Receptors, Adhesion, Secretion Part B
Tager, this series, Vol. 56, p. 214. 4 F. Rendu, M. Lebret, A. T. Nurden, and J. P. Caen, Br. J. Haematol. 52, 241 (1982). 5 M. Da Prada, J. P. Tranzer, and A. Pletscher, Experientia 28, 1328 (1972). , New York, NY), which has been used to digest briefly heart and brain tissue before homogenization. 5 M stock) and 30 mM gluconic acid 8-1actone (108 mg dissolved in 1 ml of washing medium just before addition) at 37° for 10 min. Then 3 mg of nagarse and 10 mg of ATP ~ (both dissolved in 1 ml of medium) are mixed into the suspension, which is kept at room temperature for 5 min.
S. Authi, F. Carey, and N. Crawford, Biochem. J. 222, 413 (1984). 12 N. Hack, M. Croset, and N. Crawford, Biochem. J. 233, 661 (1981). 13 K. S. Authi and N. Crawford, Biochem. J. 230, 247 (1985). 14 K. -G. Heidrich, this series, Vol. 31, p. 746. 15 K. Hannig, Electrophoresis 3, 235 (1982). 16 H. G. Heidrich and K. Hannig, Methodol. Biochem. 8, 91 (1979).  P L A T E L E T H O M O G E N I Z A T I O N BY N 2 C A V I T A T I O N 21  H o m o g e n i z a t i o n b y N i t r o g e n C a v i t a t i o n T e c h n i q u e A p p l i e d to P l a t e l e t S u b c e l l u l a r F r a c t i o n a t i o n By M.
8 j. T. Harmon and G. A. Jamieson, J. Biol. Chem. 261, 13224 (1986). 9 F. K. , "Technical Manual for the American Association of Blood Banks," 9th ed. p. 57. American Association of Blood Banks, Arlington, Virginia. 34 I S O L A T I O N OF P L A T E L E T C O M P O N E N T S  1. 7 with citric acid (1 M). 2. Platelets are sedimented at 900 g at 22 ° for 10 min (GLC-1 ; Sorvall, Newtown, CT). Due to the high concentration of platelets in the concentrates, the supernatant from the first centrifugation is recentrifuged under identical conditions and the second supernatant is discarded.